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Paediatric Pulmonology and Allergology
2005 October, Vol. VIII, No. 2 (2966-2969)
SPECTROPHOTOMETRIC ASSESSMENT OF TERATOGENITY OF OCHRATOXIN A AND MONTELUKASTUM
Antanas Januskevicius1, Povilas Gaidelis1, Arunas Valiulis2
1 Vilnius University Faculty of Medicine,
2 Clinic of Children’s Diseases of Vilnius University, Vilnius, Lithuania
It was proved on the animals that ochratoxin A is very teratogenic substance and montelukastum natricum is not teratogenic substance macroscopically, but it is still not known what changes they cause in molecular level. The purpose of our work is to investigate possible teratogenic effects of ochratoxin A and montelukastum natricum by using spectrophotometer. We injected montelukastum natricum (25 mg/kg per rat) to one group of pregnant rats in rareripe period (from the first to the eight day) of embryogenesis. The second group of pregnant rats got injections of ochratoxin A (25 pg/kg per rat) in the same period of embryogenesis. The third group got injections of physiological solution. There were taken 10 rats to everyone group. In the end of rareripe period we took a blood sample from each rat and wrote a spectrum of everyone sample by using spectrophotometer. It was found no statistically significant differences between the spectra of solutions from the first and the third group and spectra of solutions from the second group were very similar too. But it was confirmed statistically significant difference between the data of the first and third group together and the second group in the zone of 200–350 nm. At the 20 day of pregnancy was made Caesarean section to all of the rats and was found any morphologic defects of embryos of the first and third groups. At the same time, all embryos from the second group had morphologic defects. The spectra of solutions correlated with morphologic damages. We concluded that montelukastum natricum have no teratogenic effects on the embryo of rats.